Glucose-Stimulated Increment in Oxygen Consumption Rate as a Standardized Test of Human Islet Quality
Identifieur interne : 000D15 ( Main/Exploration ); précédent : 000D14; suivant : 000D16Glucose-Stimulated Increment in Oxygen Consumption Rate as a Standardized Test of Human Islet Quality
Auteurs : I. R. Sweet [États-Unis] ; M. Gilbert [États-Unis] ; S. Scott [États-Unis] ; I. Todorov [États-Unis] ; R. Jensen [États-Unis] ; I. Nair [États-Unis] ; I. Al-Abdullah [États-Unis] ; J. Rawson [États-Unis] ; F. Kandeel [États-Unis] ; K. Ferreri [États-Unis]Source :
- American journal of transplantation [ 1600-6135 ] ; 2008.
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Abstract
Standardized assessment of islet quality is imperative for clinical islet transplantation. We have previously shown that the increment in oxygen consumption rate stimulated by glucose (ΔOCRglc) can predict in vivo efficacy of islet transplantation in mice. To further evaluate the approach, we studied three factors: islet specificity, islet composition and agreement between results obtained by different groups. Equivalent perifusion systems were set up at the City of Hope and the University of Washington and the values of ΔOCRglc obtained at both institutions were compared. Islet specificity was determined by comparing ΔOCRglc in islet and nonislet tissue. The ΔOCRglc ranged from 0.01 to 0.19 nmol/min/100 islets (n = 14), a wide range in islet quality, but the values obtained by the two centers were similar. The contribution from nonislet impurities was negligible (ΔOCRglc was 0.12 nmol/min/100 islets vs. 0.007 nmol/min/100 nonislet clusters). The ΔOCRglc was statistically independent of percent beta cells, demonstrating that ΔOCRglc is governed more by islet quality than by islet composition. The ΔOCRglc, but not the absolute level of OCR, was predictive of reversal of hyperglycemia in diabetic mice. These demonstrations lay the foundation for testing ΔOCRglc as a measurement of islet quality for human islet transplantation.
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Rawson</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Diabetes, Endocrinology, & Metabolism, City of Hope National Medical Center</s1><s2>Duarte, CA</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Kandeel, F" sort="Kandeel, F" uniqKey="Kandeel F" first="F." last="Kandeel">F. Kandeel</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Diabetes, Endocrinology, & Metabolism, City of Hope National Medical Center</s1><s2>Duarte, CA</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Ferreri, K" sort="Ferreri, K" uniqKey="Ferreri K" first="K." last="Ferreri">K. 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R." last="Sweet">I. R. Sweet</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Medicine, University of Washington</s1><s2>Seattle, WA</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Washington (État)</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation></author><author><name sortKey="Gilbert, M" sort="Gilbert, M" uniqKey="Gilbert M" first="M." last="Gilbert">M. Gilbert</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Medicine, University of Washington</s1><s2>Seattle, WA</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Washington (État)</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation></author><author><name sortKey="Scott, S" sort="Scott, S" uniqKey="Scott S" first="S." last="Scott">S. Scott</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Diabetes, Endocrinology, & Metabolism, City of Hope National Medical Center</s1><s2>Duarte, CA</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Todorov, I" sort="Todorov, I" uniqKey="Todorov I" first="I." last="Todorov">I. Todorov</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Diabetes, Endocrinology, & Metabolism, City of Hope National Medical Center</s1><s2>Duarte, CA</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Jensen, R" sort="Jensen, R" uniqKey="Jensen R" first="R." last="Jensen">R. Jensen</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Medicine, University of Washington</s1><s2>Seattle, WA</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Washington (État)</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation></author><author><name sortKey="Nair, I" sort="Nair, I" uniqKey="Nair I" first="I." last="Nair">I. Nair</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Diabetes, Endocrinology, & Metabolism, City of Hope National Medical Center</s1><s2>Duarte, CA</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Al Abdullah, I" sort="Al Abdullah, I" uniqKey="Al Abdullah I" first="I." last="Al-Abdullah">I. Al-Abdullah</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Diabetes, Endocrinology, & Metabolism, City of Hope National Medical Center</s1><s2>Duarte, CA</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Rawson, J" sort="Rawson, J" uniqKey="Rawson J" first="J." last="Rawson">J. Rawson</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Diabetes, Endocrinology, & Metabolism, City of Hope National Medical Center</s1><s2>Duarte, CA</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Kandeel, F" sort="Kandeel, F" uniqKey="Kandeel F" first="F." last="Kandeel">F. Kandeel</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Diabetes, Endocrinology, & Metabolism, City of Hope National Medical Center</s1><s2>Duarte, CA</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Ferreri, K" sort="Ferreri, K" uniqKey="Ferreri K" first="K." last="Ferreri">K. Ferreri</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Diabetes, Endocrinology, & Metabolism, City of Hope National Medical Center</s1><s2>Duarte, CA</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j" type="main">American journal of transplantation</title><idno type="ISSN">1600-6135</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">American journal of transplantation</title><idno type="ISSN">1600-6135</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animal</term><term>Diabetes mellitus</term><term>Exploration</term><term>Glucose</term><term>Graft</term><term>Homograft</term><term>Human</term><term>Langerhans islet</term><term>Mouse</term><term>Oxygen consumption</term><term>Quality</term><term>Rate</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Diabète</term><term>Glucose</term><term>Consommation oxygène</term><term>Greffe</term><term>Taux</term><term>Exploration</term><term>Homogreffe</term><term>Homme</term><term>Ilot Langerhans</term><term>Qualité</term><term>Animal</term><term>Souris</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Diabète</term><term>Glucose</term><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Standardized assessment of islet quality is imperative for clinical islet transplantation. We have previously shown that the increment in oxygen consumption rate stimulated by glucose (ΔOCR<sub>glc</sub>) can predict in vivo efficacy of islet transplantation in mice. To further evaluate the approach, we studied three factors: islet specificity, islet composition and agreement between results obtained by different groups. Equivalent perifusion systems were set up at the City of Hope and the University of Washington and the values of ΔOCR<sub>glc</sub> obtained at both institutions were compared. Islet specificity was determined by comparing ΔOCR<sub>glc</sub> in islet and nonislet tissue. The ΔOCR<sub>glc</sub> ranged from 0.01 to 0.19 nmol/min/100 islets (n = 14), a wide range in islet quality, but the values obtained by the two centers were similar. The contribution from nonislet impurities was negligible (ΔOCR<sub>glc</sub> was 0.12 nmol/min/100 islets vs. 0.007 nmol/min/100 nonislet clusters). The ΔOCR<sub>glc</sub> was statistically independent of percent beta cells, demonstrating that ΔOCR<sub>glc</sub> is governed more by islet quality than by islet composition. The ΔOCR<sub>glc</sub>, but not the absolute level of OCR, was predictive of reversal of hyperglycemia in diabetic mice. These demonstrations lay the foundation for testing ΔOCR<sub>glc</sub> as a measurement of islet quality for human islet transplantation.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Washington (État)</li></region><settlement><li>Seattle</li></settlement><orgName><li>Université de Washington</li></orgName></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Sweet, I R" sort="Sweet, I R" uniqKey="Sweet I" first="I. R." last="Sweet">I. R. Sweet</name></region><name sortKey="Al Abdullah, I" sort="Al Abdullah, I" uniqKey="Al Abdullah I" first="I." last="Al-Abdullah">I. Al-Abdullah</name><name sortKey="Ferreri, K" sort="Ferreri, K" uniqKey="Ferreri K" first="K." last="Ferreri">K. Ferreri</name><name sortKey="Gilbert, M" sort="Gilbert, M" uniqKey="Gilbert M" first="M." last="Gilbert">M. Gilbert</name><name sortKey="Jensen, R" sort="Jensen, R" uniqKey="Jensen R" first="R." last="Jensen">R. Jensen</name><name sortKey="Kandeel, F" sort="Kandeel, F" uniqKey="Kandeel F" first="F." last="Kandeel">F. Kandeel</name><name sortKey="Nair, I" sort="Nair, I" uniqKey="Nair I" first="I." last="Nair">I. Nair</name><name sortKey="Rawson, J" sort="Rawson, J" uniqKey="Rawson J" first="J." last="Rawson">J. Rawson</name><name sortKey="Scott, S" sort="Scott, S" uniqKey="Scott S" first="S." last="Scott">S. Scott</name><name sortKey="Todorov, I" sort="Todorov, I" uniqKey="Todorov I" first="I." last="Todorov">I. Todorov</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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